Products

IRIS dyes are fluorescent innovative dyes belonging to the well-known family of cyanine dyes.

Cyanine technologies manufactures cyanine dyes emiting in the range of the spectrum between 500 and 850 nm (Visible and NearInfraReed, NIR)

IRIS dyes are available as free dye, NHS esters and conjugates with the most commonly used biomolecules in molecular biology and fluorescent imaging.

Secondary antibodies, oligonucleotides, strepavidin and other proteins labelled with the desired IRIS dye are available in stock. and non standard conjugates with other molecules are available on demand.

Please contact us for information about research, products and for quotations.

IRIS Dyes labelled nucleotides can be used as labels for DNA and RNA with the most widely known methods such as nick translation, random priming, polymerase chain reaction, cDNA synthesis etc.

The fluorescent analogs are purified by HPLC chromatography. Analytical HPLC is used as a quality control check to ensure chemical purity >95%. UV/VIS absorption spectra are obtained in aqueous phosphate buffer to determine concentration.

IRIS dyes labelled dUTP, dCTP, UTP and CTP can be enzymatically incorporated into probes for use in many molecular biology applications. The use of fluorescent nucleotides with different fluorescence emission profiles allows multicolor applications. We have developed the following protocols for incorporating these fluorescent nucleotides into hybridization probes. Depending on your application, some further optimization of the reaction conditions may be necessary.